Do you really know the accuracy of cell counters?
The word “accuracy” is often used when someone is describing how well a device performs. But frequently the term accuracy is not used properly. Often it is used interchangeably with precision and trueness. These terms may seem similar but have very different meanings. So, what is accuracy? What is trueness? What is precision?
In the whitepaper "The accuracy of cell counters" these terms are explained and how they relate to cell counters. Using the CytoSMART Exact as a clear example, tools are proved to distinguish between claims made by laboratory equipment manufacturers and aid you in using the correct terminology in your research.
The whitepaper goes into detail on how to determine the accuracy of a cell counter. It showes that accuracy is made up of two components: trueness and precision. We illustrate how these are related to the performance of a cell counting device.
For the trueness of a device, always focus upon R2 given. This value should always be close to one. But make sure to inquire if the value presented is the adjusted R2. Otherwise by using an unadjusted R2 one may mask bad results. Regarding precision, you should look for the CV value given of the device. The CV value should always be lower than the theoretical value. Note here that the CV value should be derived from a large sample size, with N > 5.
After reading the whitepaper "The accuracy of cell counters" you will know what to look for when reading tech-notes describing. You know what information provided is most important when looking for a new cell counter.